Isolation of a Urea Degrading Bacteria :: Biology

Isolation of a Urea Degrading Bacteria Introduction Urea was the first organic chemical to be synthetically produced1, previously it was thought that only living creatures could produce organic compounds Urea is naturally produced by the kidneys as waste from the degradation of amino acids. It is because of this that urea is commonly found in soils and is a useful nutrient source for bacteria that are able to utilise it, such as, Helicobacter pylori, Klebsiella pneumonia, all species of Proteus and Micrococcus luteus. These bacteria degrade urea in a reaction catalysed by the urease enzyme, CO(NH2)2 + H2O Ã  CO2 + 2NH3. this process benefits the bacteria in several ways. The bacteria use the ammonia that is produced for respiration, the products also raise the pH of the environment. This promotes the growth of many urea degrading bacteria and inhibits competition from many other bacterial species. M. luteus is commonly found on mammalian skin and it is unusual for a member of the natural human flora to degrade urea. It is believed that M. luteus has this ability as an evolutionary hangover from its life in its ancestral soil habitat. In this environment urea is readily available and the ability to degrade it is a distinct advantage. As the species evolved to live on skin the trait remained, as it had no negative effect on survivability. Micrococcus is a genus within the Micrococcaceae family. With the use of 16s RNA in bacterial taxonomy the genus has recently been revised2. The genus now includes three species, M. luteus, M. lylae and M. antarcticus3. M. luteus is a common yellow gram-positive coccus and roughly 0.5-2.0mm in diameter. Cells appear in pairs, tetrads and irregular clusters but never in chains.4 Method of Isolation * Isolate a variety of organisms from soil and skin. By taking samples from four different sources (three skin and one soil) the chance of urea degrading bacteria being present was increased. * Culture in nutrient broth. This allowed all isolated microbes to grow. * Plate sample onto urea plates. On these plates urea was the only nutrient available, this meant that any bacteria that grew could degrade urea. * Perform urease test. Isolated bacteria are grown in a broth containing phosphate buffer, yeast extract, 2% urea and phenol red. An agar slope of the medium is heavily inoculated and incubated at 370c for at least four hours. If the organism only has low urease activity the phosphate buffer will neutralise the NH3 produced. A red colour indicates that NH3 has been produced and the result is positive5. * Perform Gram stain. This is the most important stain in bacteriology and differentiates between gram positive and gram-negative cell walls, which indicates

Irony in the Scarlet Letter Essay

â€Å"Irony regards every simple truth as a challenge. † When reading a story, the events that have already presented themselves, lead a person to perceive what is going to happen, but when that person encounter an unexpected event, as commonly experienced through irony, it changes what the person perceives is going to happen. The Scarlet Letter exemplifies this use of irony to challenge truth. Hawthorne provides details about a specific character, but then creates an event which stands in contrast to these details. Hawthorne’s uses irony, portrayed through characters’ names, the first scaffold scene, and the Puritan community, to express the truth throughout the novel. In The Scarlet Letter, Hawthorne uses characters’ names to contrast to their actual characteristics. He uses the name Pearl, which means purity, as a nickname for a mischievous character. He portrays Roger Chillingworth as a doctor, while Chillingworth’s main purpose involves causing the deterioration of Arthur Dimmesdale’s health. Arthur Dimmesdale, a saint-like figure to the Puritan Community, indulges in a great sin. Being a minister, his life elucidates hypocrisy. He has committed one of the greatest sins that he condemns in his sermons. Hawthorne uses these evident labels to contrast to character’s true characteristics. The scaffold scenes each reveal a truth through use of irony. The first scaffold scene connotes not only a connection between Hester and Dimmesdale, but also Dimmesdale’s wishes in regard to their sin. At the beginning of the novel, while the reader’s main question involves Pearl’s father, Hawthorne asides other characters by emphasizing Dimmesdale’s questioning of Hester. This emphasis exposes Dimmesdale as the prime suspect to be Pearl’s father. Dimmesdale speaks curiously in third-person about what Pearl’s father should do. He also stresses that Hester should tell who she had an affair with, and that her partner will accept being exposed, as if trying to convince her that he wants to be revealed but is to scared to do so on his own. This event causes irony, as the focus on Dimmesdale and Hester in this scene foreshadows their relations later in the novel. Hawthorne portrays the Puritan Community as a body that lacks the ability to recognize truth, while their ideals involve creating a â€Å"city upon a hill† that has achieved the ultimate truth. When Roger Chillingworth arrives in Boston, the community falsely believes that he has been sent from God to cure Arthur Dimmesdale. When Chillingworth wishes to house with Dimmesdale, few question Chillingworth’s intent. Even as they see Dimmesdale’s worsening condition, few blame it on Roger Chillingworth. The community also fails to recognize Dimmesdale’s attempts to confess his sin. In his sermons, Dimmesdale states that he is â€Å"altogether vile, a viler companion of the vilest† and that he should be â€Å"shriveled up before their eyes by the burning wrath of the Almighty. The community, still believing that he has not committed any serious sin, thinks of him even the higher. The community’s inability to recognize evil characters and sin overshadows their wishes to perceive the ultimate truth. Characters’ names, the first scaffold scene, and the Puritan Community act as a means of expressing truth through Hawthorne’s use of irony. Hawthorne provides evident details on characters, allowing the reader to obtain truth through an ironic event. Hawthorne meets the quote in the beginning by the requirement that irony should regard every simple truth as a challenge.

Model Device Security to Keep Ones Data Safe - 687 Words

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The first segment will consist of the Problem Statement, it will encompass the background information, causes, business impacts, a cost and risk analysis, any assumptions, and what limitations, if any the problem faces. A list of technical terms is provided for reference as well. The second segment will consist of the Solution technology provides and will provide what guidelines should be followed in order to solve the said problem. It will encompass the areas of business drivers, justification, what the effects are for choosing not to implement the solution, and a detailed description of the preventative solution. Problem Statement Mobile devices typically lack the security featuresShow MoreRelatedPrivacy And The Right Of Privacy Essay1057 Words   |  5 Pagesto access to information, individual’s privacy. There is free flow of data and information on web which provides a great threat to the right of privacy. 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